Banca de DEFESA: KEITY LAIANE GOMES TRINDADE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KEITY LAIANE GOMES TRINDADE
DATE: 14/02/2023
TIME: 17:00
LOCAL: UFRPE - remota
TITLE:

USE OF RAPID BLOOD TEST IN EQUINE FACES

KEY WORDS:

Well-being, diagnosis, equestrian sport, intestinal injury, gastric ulcer

PAGES: 87
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

Diseases in the gastrointestinal tract (GIT) of horses have been reported as frequent health problems, leading to economic losses for livestock. Among the diseases of the GI tract, gastric (UG) and large colon (UCM) ulcers are the most frequent; however, only the UG have a precise diagnostic method, performed through endoscopies. Recently, rapid tests were developed to identify fecal occult blood in horses, seeking to identify possible intestinal lesions. Thus, the present study aimed to correlate clinical and laboratory signs of gastrointestinal diseases with the findings of rapid fecal evaluation tests. 155 animals from different breeding, maintenance (NPE+CVA) and training systems for different sports (racing, walking and vaquejada) were used. The feces were obtained through natural defecation, stored in clean containers, for later analysis through the rapid test (SUCCEED®), following the manufacturer's recommendations; in addition to fecal pH measurement directly on fresh feces using a portable pH meter (OAKTON 50S). Blood samples were obtained by collecting them in vacuum tubes containing EDTA, for carrying out blood counts in an automatic cell counter (Roche® Poch 100iv) obtaining: red blood cells (HE), hemoglobin (HB), hematocrit (HT), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), erythrocyte distribution range coefficient of variation (RDW-CV), erythrocyte distribution range standard deviation (RDW-SD), leukocytes (LEU), lymphocytes (LINF), platelets and other white cells. For biochemical analyses, samples were collected in tubes without anticoagulant, centrifuged to obtain serum for plasma protein (PPT), albumin (ALB) and gammaglutamyltransferase (GGT) analysis, carried out in semiautomatic equipment (Doles® D-250) using commercial kits and following the manufacturer's recommendations. Fibrinogen was analyzed using the thermal presipitation technique. Serum was also analyzed by ELISA method using commercial kits for calprotectin analysis. The results obtained were analyzed using the SigmaPlot 13.0 program for Windows® (Systat Software, Inc), using ANOVA and Tukey's test with a significance level set at 5%. The animals presented 16% of positive results for the presence of hemoglobin in the feces, 32% for the presence of albumin, 9% for hemoglobin and albumin and 39% for one of the markers. There was an interaction between positive results and the age of sport animals (p<0.05), which may indicate stress at the beginning of training. There was no interaction between test results and blood biomarkers (p>0.05). There was difference in the stool scale between sports (p<0.05), due to the type of food. The results obtained in the test for detecting blood in the feces, combined with the results of health biomarkers, can contribute to a better understanding of some digestive processes and the well-being of horses

COMMITTEE MEMBERS:
Presidente - HELIO CORDEIRO MANSO FILHO
Externa à Instituição - MONICA MIRANDA HUNKA - UFRPE
Externa à Instituição - SILVIA ROBLES REIS DUARTE