Banca de QUALIFICAÇÃO: CID ARISTOTELIS DE SIQUEIRA ALENCAR

EVALUATION OF COMMERCIAL IMMUNOENZYMATIC ASSAYS APPLIED TO THE SERODIAGNOSIS OF INFECTIOUS ANEMIA EQUINE

Horse, AIE, IDGA, ELISA, diagnosis.

Equine infectious anemia (EIA) is a disease of cosmopolitan distribution that affects the equine family. As the disease does not have an efficient treatment or vaccine, it depends on diagnostic tools to take precise health measures. The objective of this study is to evaluate the competitive enzyme immunoassays (competitive cELISA Enzyme Linked Immunosorbent Assay) and the ELISAi (indirect Enzyme Linked Immunosorbent Assay) used for the diagnosis of EIA. There were 846 equine sera samples. A precision study was performed to assess repeatability through standard deviation estimation and calculation calculation. The performance of the ELISAi and CELISA kits was evaluated based on their sensitivities and agreement in their specificities with AGID. The ELISAi presented a sensitivity of 96.89% and a specificity of 91.85%, while the ELISA presented a sensitivity of 98.46% and a specificity of 94.79%. Both showed an excellent agreement rate (Kappa = 08) with the AGID test. Among the ELISA tests, in addition to having a small advantage in terms of specificity and sensitivity, cELISA has in favor even a shorter test run time (45 minutes) compared to ELISAi, which is 2 hours. The results obtained were evaluated and the results obtained were evaluated, with the lowest results obtained, 3 and 11.9 evaluated. Calculated from the positive repeat of positive and negative sera and internal control, analyzed between and interplates. ELISA tests over ELISA with advantages such as AGID tests, such as speed of result, automation, the possible ELISAs of proposed EL tests, have high sensitivity and proposed ELISAs, compatible with the role to which it was proposed.