Banca de QUALIFICAÇÃO: RAFAEL ARTUR DA SILVA JÚNIOR

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : RAFAEL ARTUR DA SILVA JÚNIOR
DATE: 15/08/2022
TIME: 16:00
LOCAL: Auditório da Área de Reprodução Animal do Departamento de Medicina Veterinária
TITLE:

The beneficial effects of antifreeze protein from Lolium perenne (LpAFP) in the vitrification of in vitro-produced bovine embryos

KEY WORDS:

Cryopreservation; blastocysts; IVF; Assisted reproduction; cryotop.

PAGES: 61
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

Embryo cryopreservation has become an indispensable tool in livestock, especially in the genetic improvement of herds. The use of cryoprotective molecules, such as antifreeze proteins (AFP), is useful in reducing cryodanes and improving this technique. In the present study, the cryoprotective effects of Lolium perenne antifreeze protein (LpAFP) on the vitrification of bovine embryos were evaluated. For this, in vitro produced blastocysts (n=120) were divided into two groups: Control Group (GC) without addition of LpAFP and the Treatment Group (GT) with addition of 500 ng/mL of LpAFP in equilibrium and vitrification media; and vitrified using cryotop. Vitrification was carried out by transferring the blastocysts to the equilibrium solution: 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 2 min, then to the vitrification solution: 15% EG, 15 % dimethyl sulfoxide (DMSO) and then deposited on the cryotop rod and submerged in liquid nitrogen. Heating was carried out in three steps with decreasing concentrations of sucrose (1.0, 0.5 and 0.5 M sucrose). The results showed that there was no significant difference in the reexpansion rate 24 hours after heating, however there was variation (P < 0.05) in the hatching rate in the GT. The total number of cells 24 hours after heating was significantly higher in the GT when compared to the GC (TG 114.87 ±  7.24 vs. GC 91.81 ± 4.94). In conclusion, the addition of 500 ng/mL of LpAFP during the vitrification of in vitro produced bovine embryos was shown to be favorable in improving the survival and development of blastocysts after heating, suggesting better pregnancy outcomes for in vitro produced and cryopreserved embryos.

BANKING MEMBERS:
Presidente - 3085776 - ANDRE MARIANO BATISTA
Interno - 1511486 - CLAUDIO COUTINHO BARTOLOMEU
Externa ao Programa - 1957902 - ANDREIA FERNANDES DE SOUZA - UFRPE