Banca de DEFESA: DIÊGO LUCAS COUTO PAES BARRETO DE CARVALHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DIÊGO LUCAS COUTO PAES BARRETO DE CARVALHO
DATE: 25/02/2022
TIME: 09:00
LOCAL: Videoconferência: https://meet.google.com/ryb-zeko-vvw
TITLE:

Morphological, morphometric, histopathological and thermographic evaluation of skin flaps treated with phytotherapy ointment of Anthurium affine Schott in rats

 

 

KEY WORDS:

 Wound, wild anthurium, herbal medicine, infrared radiation.

PAGES: 82
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SUMMARY:

The objective of this work was to evaluate, by infrared thermography, morphometry and macro and microscopic morphology, the healing activity of the ointment composed of hydroalcoholic extract of Anthurium affine Schott in dorsal cranial-based random cutaneous flaps in male Wistar rats (n=18), with 90 days of age and randomly divided into three groups: Absolute Control Group (GCA) – animals without treatment (n=06); Control Group (GC) - animals treated with the ointment base (n=6); Treated Group (TG) – animals treated with A. affine Schott ointment (n=6). Each animal was submitted to the making of 8 cm long by 2 cm wide skin flaps. The GC and GT received treatment every 24 hours for 7 days. All groups were evaluated with thermography after shaving, after surgery and every 24 hours until the eighth day, when they were euthanized. On the last day of the experiment, the wounds were measured with a digital caliper to assess the area of necrosis. The histological sections of the cranial, medial and caudal portions of the flaps were stained with Hematoxylin-Eosin and Trichrome of Gomori for morphometric and morphological analysis by quantification of blood vessels and determination of collagen structuring. The data obtained were submitted to the ANOVA test complemented by the Tukey test (p<0.05). Infrared thermography has been shown to be an effective method for evaluating areas of skin flap necrosis. Treatment with ointment containing hydroalcoholic extract of A. affine Schott favored angiogenesis in the caudal portion of the flaps in GT. However, we emphasize that further studies are needed with different concentrations of the extract.

 

 

BANKING MEMBERS:
Externa ao Programa - 1786867 - DANIELA MARIA BASTOS DE SOUZA
Presidente - 383965 - JOAQUIM EVENCIO NETO
Externa à Instituição - LIGIA REIS DE MOURA ESTEVÃO