Banca de DEFESA: BRUNO HENRIQUE DE ALBUQUERQUE PAIVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRUNO HENRIQUE DE ALBUQUERQUE PAIVA
DATE: 28/02/2022
TIME: 15:00
LOCAL: REMOTO - GOOGLE MEET (meet.google.com/hxo-pzyy-dtu)
TITLE:

TRANSSURGICAL ANATOMOPATHOLOGY IN HEAD AND NECK TUMORS OF DOGS AND CATS

KEY WORDS:

Excision margins. Frozen section biopsy. Histopathology. Neoplasm.

PAGES: 70
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

Although intraoperative anatomopathological assessment has been a reality in medicine since the beginning of the last century, and its accuracy approved for use in animals, little applicability has made in veterinary medicine. Specifically, head and neck neoplasms are focus of emerging specialties in small animal medicine like ophthalmology and dentistry, which denotes an urgency in investigating the applicability of the technique in these places. The present study aimed to investigate the accuracy of the histological technique of intraoperative frozen section in diagnosing neoplasms and to evaluate the involvement of surgical margins in neoplastic processes in the head and neck of dogs and cats. Thirty-eight tumor excision surgical procedures were monitored between February and December 2021in the surgical routine of veterinary ophthalmology service, as well as in the technicians and residents in the Veterinary Hospital of Universidade Federal Rural de Pernambuco (HOVET-UFRPE). The samples has submitted to frozen section technique of histopathological evaluation and the results has compared with traditional technique of anatomohistopathological diagnosis. Adaptations to the freezing cutting technique were carried out through the use of dry ice, a by-product of dry ice manufacture, which was used both to improve the macroscopic approach of the samples, and as a method of microtomy refrigeration. The methodology used also made use of a portable sliding microtome to replace the cryostat. In total, 29 canines and 9 felines participated in this research, which 57 tumor structures has excised and evaluated, in addition to 79 analyzes of intraoperative margins, between lateral and deep margins. Analyzing the frequency, the most common sites of occurrence were 25 lesions in the eye, 12 in the neck and six in the face, while the most frequent morphological diagnoses were 11 sebaceous adenomas, 9 squamous cell carcinomas and 5 mast cell tumors. An accuracy observed of 94.8%, with a sensitivity of 95.5% and a specificity of 94.3% and a kappa agreement index of 0.9 for the ability to distinguish between benign and malignant processes. Regarding the ability to distinguish between neoplastic and non-neoplastic processes, an accuracy of 91.23%, sensitivity of 90.9% and specificity of 92.3% has identified. For the analysis of intraoperative margins, good results has also observed, with an accuracy of 91.14%, specificity of 93.22% and sensitivity of 85%. These findings are superior to studies carried out in both medicine and veterinary medicine, indicating that the transsurgical anatomopathology of head and neck tumors using dry ice and freezing microtome is an applicable, fast, simple and low-cost alternative, improving follow-up of cancer patients.

BANKING MEMBERS:
Presidente - 1929164 - FRANCISCO DE ASSIS LEITE SOUZA
Externo à Instituição - BRENO SOUZA SALGADO - UFES
Externo à Instituição - ROBERIO SILVEIRA DE SIQUEIRA FILHO - UFRPE