Banca de DEFESA: JOSÉ PETRÔNIO MENDES JÚNIOR

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JOSÉ PETRÔNIO MENDES JÚNIOR
DATE: 08/04/2024
TIME: 14:00
LOCAL: Prédio Prof. Mateus Rosas Ribeiro
TITLE:

Microbiome of the nickel hyperaccumulators Lippia lupulina and Justicia Lanstyakii endemic to the ultramafic soils of Niquelândia

KEY WORDS:

Meta-analysis. Amplicon sequencing. Illumina. Serpentine soils. Rhizosphere. Bioinformatics.

PAGES: 125
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Ciência do Solo
SPECIALTY: Microbiologia e Bioquímica do Solo
SUMMARY:

The ultramafic complex in Niquelândia, Goiás, hosts Brazil's largest nickel reserve and is home to endemic plant species capable of accumulating over 1000 mg kg-1 of Ni in their leaves, known as nickel hyperaccumulators. These plants represent a sustainable alternative to ore extraction. The microbiome plays a fundamental role in plant adaptation to the environment, acting as an amplifier of their adaptive capacity. The aim of this study is to investigate the microbiome of the nickel hyperaccumulator plant species Justicia lanstyakii and Lippia lupulina in the ultramafic complex of Niquelândia, using Illumina amplicon sequencing. Additionally, data published in the last five years were analyzed for a meta-analysis of the microbiome in ultramafic soils worldwide, aiming to identify a possible microbial signature in these  environments. A total of 1080 ultramafic soil samples were analyzed globally through meta- analysis, using high-throughput Illumina sequencing data. Research articles available in the  Scopus and Web of Science databases were selected for this analysis. Moreover, amplicon sequencing was performed to investigate the microbiome associated with the rhizosphere of the nickel hyperaccumulator plant species Justicia lanstyakii and Lippia lupulina from the ultramafic complex of Niquelândia, as well as the bulk soil of the plants. Our results revealed significant diversity of phyla, including Acidobacteria, Actinobacteria, Chlorobi, Chloroflexi, GAL15, Mixococota, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia, highlighting the richness of these soils. Bulk soil samples showed a predominance of phyla such as Acidobacteriota (23.5%), Spirochaetota (19%), and Chloroflexota (15.93%). In the rhizosphere of J. lanstyakii, the main associated phyla were Actinobacteriota (35.57%), Acidobacteriota (25.5%), and Verrucomicrobiota (17.97%), while in L. lupulina, they were Actinobacteriota (34.73%), Acidobacteriota (21.07%), and Verrucomicrobiota (13.17%). This study highlighted not only the diversity and structure of these microbiomes but also emphasized the notable enrichment and formation of more complex interactions.

COMMITTEE MEMBERS:
Externo à Instituição - LUCAS WILLIAM MENDES - USP
Externo à Instituição - ARTHUR PRUDÊNCIO DE ARAÚJO PEREIRA - UFC
Externo à Instituição - FELIPE MARTINS DO RÊGO BARROS - USP
Externo à Instituição - FERNANDO BRUNO VIEIRA DA SILVA - UFRPE
Presidente - GISELLE GOMES MONTEIRO FRACETTO