MICROBIAL ANALYSIS AND POTENTIAL USE IN AGROECOLOGICAL SYSTEMS OF HONEY FROM Trigona spinipes – Fabricius - 1793
Stingless bee, Arapuá, Bacillus, Bioprospecting, Caatinga
Native stingless bees or meliponines represent an important portion of Brazil's biodiversity. Such insects constitute an element of indispensable ecological importance in preserving the floral diversity of multiple Brazilian ecosystems. This category of insects, genuinely adapted to Brazilian biomes, consists of approximately 300 species of melipones classified into different genera. They produce honey with many peculiarities to be discovered. In addition to having features such as adaptations to prevent the deterioration of your food containers. Due to this mechanism, the various species of native bees have potential relationships with several already identified microorganisms. In this work, samples of Trigona spinipes honey collected in the Território do Sertão do São Francisco-PE were analyzed. The findings included six filamentous fungi, seven yeasts and fourteen bacteria. All analyzes were performed in triplicate and micro and macroscopic observation techniques and Gram staining were performed. The genus Bacillus was most frequent in bacteriological findings. For specific identification of each isolate, analyzes were carried out using molecular biology. The results obtained provide precedents for more detailed analyzes of the composition of microorganisms associated with Trigona spinipes and other species of stingless bees from the Caatinga, as the various species of native bees have potential relationships with several already identified microorganisms. These may be bioremediation relationships, use as bioinsecticides due to their entomopathogenic action, in bioprospecting and even as bioinputs that can assist agroecological practice. Preserving Arapuá certainly means providing sustainability for the various ecosystems, as it is a species that repairs degraded biomes